Protein Method Blocking Buffers
Research Products International Corp Blotto-Tween 20 Blocking Solution in TBS with Azide, 500 Milliliters
Solution contains:
150mM NaCl
3% BSA
0.05% Tween-20
0.02% Sodium Azide
Optimized for use in western blotting to minimize non-specific binding of antibodies.
pH: 7.4 ± 0.2
Seracare Life Sciences Inc 10X TBS W/ 0 5% TWEEN 20(TBST)
10X Tris-buffered Saline (TBS) with 0.5% Tween 20 is used as a common Western blot wash solution and general wash buffer in immunoassay applications. Tween 20, a non-ionic detergent, reduces non-specific binding and protein-protein interaction during the wash step in immunoassay procedures.
Bioworld Casein-Blocking Buffer in PBS w/ Azide, 500 mL
Product DescriptionCasein-blocking buffer is 3% in PBS with 0.02% sodium azide. It is a liquid stored between temperatures of -30°C to -10°C, and is used for Western blot and ELISA. Buffer used for blocking excess binding sites on membranes and microplates in antibody-based detection methods.
Bioworld Casein-Blocking Buffer in TBS, 1 L
Casein Blocking Buffer in TBS Blot blocking buffer used to block non-specific binding site for antibodies on the membrane. Technical Information: 1% Casein in TBS
Quality Biological Inc DPBS with TWEEN 20 without Ca & Mg, 10 Liters
DPBS can be used to provide a buffer system to maintain cell culture media in the physiological pH range of 7.0 to 7.6. ¿ DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ DPBS is also used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate, to wash the wells of microtiter plates, and to wash cells during preparation and serial transfer. ¿ 1X working solution is recommended ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
ILEX LIFE SCIENCES LLC Block Ace Powder: blocking reagent for immunological experiments (1 x 40 g)
Block Ace Powder: blocking reagent for immunological experiments. Block Ace is a blocking agent prepared from bovine milk proteins (lactoprotein). Since lactoprotein contains a lot of hydrophobic groups, it shows a high affinity to plastic, membrane filter, etc. Block Ace exhibits a superior blocking effect on nonspecific adsorption of antigens or antibodies in ELISA, RIA and immunoblocking methods compared with conventional blocking agents such as BSA (Bovine Serum Albumin). FEATURES: Since Block Ace has high blocking effects and maintains the background at a low level, it is useful for low-concentration measurement such as ELISA. Block Ave is heat-treated and thus bovine-derived antibodies, enzymes and viruses are inactivated. Block Ace is stable for heat and freezing. It can stabilize physiologically active substances and can be used as a diluting agent. Block Ace can be rapidly dissolved in water. MANUFACTURER: KAC Co., Ltd., Kyoto-city 603-8432, Japan.
Research Products International Corp TBS with Tween, 20X, 7.4 pH, Sterile Buffered, 1 Liter
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is a commonly used buffer solution in the life science marketplace. TBS stands for Tris-Buffered Saline, and Tween refers to Tween 20, a nonionic detergent.
TBS with Tween is primarily used in various applications such as Western blotting, immunohistochemistry, and antibody-based assays. It serves as a buffer for sample dilution, antibody washing, and blocking steps during these experimental procedures.
The buffer solution is prepared at a 20X concentration, meaning it is twenty times more concentrated than the final desired concentration. This concentrated form allows for efficient storage and transportation.
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is specifically formulated to have a pH of 7.4, which is the physiological pH of many biological systems.
Genetex Inc GeneTex Blocking Buffer, 5% Milk For WB and ELISA, Reduces Background Interference, 250 mL
GeneTex Blocking Buffer, TBS with 5% Milk is a ready-to-use solution that enhances assay sensitivity by minimizing non-specific background interference in immunological detection methods. It is specifically validated for Western blotting (WB) and ELISA, improving signal-to-noise ratios by binding to sites of nonspecific interaction. This liquid formulation comes in a 250 mL volume, making it ideal for various applications including immunohistochemistry.
INNOVEX BIOSCIENCES UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 30 ML
Innovex peptide-based Fc Blocker universally blocks all types Fc receptors (I, II, III) of human, mouse & all animal species; Contains NO antibody, Ig or Ig fragments, is used for IHC, IF & flow cytometry.
LAB BIOREAGENTS Strongblock I 500ML
Lab Bioreagents StrongBlocks (I, II, III) are newly developed blocking buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays The blocking buffer is to block excess binding sites on the micro-plate for reducing non-specific binding signal, by which the assay sensitivity and specificity are improved. As many factors can influence nonspecific binding, there is no single blocking buffer that is ideal for each assay. For example, depending on the recognition of detection probes from different assays, one individual blocking buffer may cross-react with detection probe. StrongBlocks (I, II, III) are a variety of buffers designed by different blocker components. During the assay, the test different blocking buffers helps to develop a immunoassay with low background noise and high signal-to-noise.
SCANTIBODIES LABORATORY INC HETEROPHILIC BLOCKING TUBE (3IX762) 50PK
The 3IX762 HBT (Heterophilic Blocking Tube) from Scantibodies Laboratory, Inc. contains a unique blocking reagent composed of specific binders which inactivate heterophilic antibodies. Once the specific binders have bound to the heterophilic antibodies, the antibodies are no longer able to cause immunoassay interference. The reagent is in the form of a lyophilized pellet at the bottom of the tube and each tube contains enough reagents to inactivate the heterophilic antibodies in 500 μl of sample material. The HBT also allows for the rapid and simple elimination of false positive heterophilic interference in plasma or serum for immunoassays (i.e., FSH, LH, Prolactin, TSH, Ferritin, CEA, AFP, hCG, HBsAg, CK-MB, CA 125, CA 19-9, NSE, etc.).
Boca Scientific Inc The Blocking Solution, 500ml
The Blocking Solution is a high-performance blocking reagent designed for saturating free binding sites on plastic surfaces or other protein binding surfaces and for minimizing nonspecific binding to surfaces in immunoassays. It is ideal for assays that require high reproducibility and low coefficients of variation, such as assays for pre-clinical and clinical studies, production of high quality diagnostics or measurement of precious specimens. The Blocking Solution is based on highly purified casein that has been chemically modified for maximum adsorption efficiency and a dense blocking layer. By incubating a surface with The Blocking Solution, such as a microtiter plate or western blotting membrane, free spaces on the surface are saturated by the components of this blocker. This minimizes unwanted binding of the analyte or the detection antibody to the surface, avoiding background and improving sensitivity of the assay.