Protein Method Blocking Buffers
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting 3-PACK (3 x 500 ml) - MB-070-003
Western Blot Blocking Solution is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Western Blot blocking buffer is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Abcam 10 x Blocking Buffer, 50ML
10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative
The product is subject to the following: Abcam Restricted Use Statement
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (20X, with 1% Tween-20, pH 7.4) - 4x1L
20X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.
Biogenex Laboratories Power Block
Power Block ™ reagent is a highly effective blocking reagent capable of reducing non-specific background staining associated with immunodetection techniques such as immunocytochemistry, ELISA, and Western blotting. Antibodies tend to bind non-specifically to membranes and tissues. To minimize this nonspecific binding, a blocking buffer is used to enhance signal-to noise ratio. Power Block™ reagent shows no cross-reactivity and it blocks more effectively than serum albumins. The blocking potency of Power Block™ reagent is superior to most other conventional formulations in producing a high signal-to-noise ratio. Power Block™ reagent may also be used as effective diluents for antibodies.""""
Vector Laboratories Animal-Free Blocker and Diluent (AFB), Ready-to-Use (R.T.U.) 100 ml
R.T.U. Animal-Free Blocker and Diluent is a ready-to-use antibody diluent and blocking reagent that stabilizes diluted antibody solutions and diminishes background due to non-specific binding in immunohistochemical applications. It contains no animal-derived proteins and can be used as an alternative to sera, BSA, casein or non-fat dry milk in situations where these or other traditional blocking agents are ineffective. It is especially convenient for multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.
INNOVEX BIOSCIENCES UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 5 ML
UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 5 ML
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting - MB-070
Western Blot Blocking Solution is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Western Blot blocking buffer is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Bioworld Casein Blocking Buffer with Fish Gelatin, 100 mL
Casein Fish Gelatin Blocking Buffer is a non-mammalian blocking solution that does not cross-react with mammalian antibodies and is suitable for ELISA assays. Blocking buffer is supplied in a 10X concentration of physiological saline buffer. Technical Information: Casein Blocker with Fish Gelatin is a casein-based blocking agent Includes 3 X 30 mL, 10X concentratePrepares 900ml of working solution1X working solution contains: 1% casein, 0.45% fish gelatin in PBS.
Cell Signaling Technology Tris Buffered Saline with Tween 20 (TBST-10X) 1000 ml
Tris Buffered Saline with Tween 20 (TBST-10X) 1000 ml
Revvity Health Sciences Inc AlphaLISA HiBlock Buffer (10X), 10 mL
AlphaLISA HiBlock Buffer (10X), 10 mL
Alkali Scientific CellPro™ PBS 20X + Tween 20, 1 L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Electron Microscopy Sciences Aurion R-Gent SE-EM Kit 30 ML
AURION R-Gent SE-EM is a high efficiency silver enhancement reagent for electron microscopy. It intensifies the gold particles by homogeneous deposition of metallic silver on the particle surface. The resulting electron dense signal is easily detected and is compatible with heavy metal counterstaining. The reagent has extremely delayed auto-nucleation and can be used under standard laboratory light conditions. It also has low viscosity, which is especially advantageous for pre-embedding immunogold applications.
Bioworld ELISA Reaction Buffer, 50mM Carbonate-Bicarbonate, 1 L
Product DescriptionELISA reaction buffer has a 50 mM concentration of carbonate-bicarbonate and a pH level of 9.6. It contains 1.59 g Na2CO3, 2.93 g NaHCO3, and dissolved in 1 liter of deionized water.Carbonate/bicarbonate buffer at a pH greater than 9 is used for the attachment of an antigen or of an antibody onto an ELISA plate. Direct coating of ELISA is an antigen immobilization technique, in which antigens are directly attached to the plate by passive adsorption. Most but not all proteins bind tightly to the polystyrene surface of microplates in alkaline conditions. Indirect coating of ELISA is another antigen immobilization technique in which antigen-specific antibodies are adsorbed onto the plastic, which in turn binds and immobilizes the antigen upon incubation with the antigen sample. Both techniques typically use the same buffer.